Figure 2

Circular Dichroism spectra analysis of (A) His-Z _HER2 -BNC and (B) GALA-His-Z _HER2 -BNC. Circular dichroism (CD) measurements were carried out with a J-725 K (JASCO, Japan). Spectra were obtained using 0.5 nm bandwidth, a scan rate of 20 nm/min and a response time of 4 sec. The quartz cuvette pathlength was 2 mm. The CD measurements were made using protein concentrations of 0.1 mg/ml and performed at 20°C. The gray circle and the black square show the CD measurements of BNCs after incubation of 1 h in PBS at pH 7.4 and 5.0, respectively.