Figure 4
Cell viability of SKBR3 and HeLa treated with LP (black bars), His-Z HER2 -BNC/LP (gray bars) and GALA-His-Z HER2 -BNC/LP (white bars). Approximately 5 × 103 SKBR3 or HeLa cells were seeded in 96-well plate. LP, His-ZHER2-BNC/LP and GALA-His-ZHER2-BNC/LP containing calcein (3.75 μl) were added to 100 μl of the medium and then the cells were cultured for 1 h. After washing with serum-free medium twice, cells were incubated with FBS-containing medium for 5 h, 23 h and 47 h. Cell viability was measured using Cell Counting Kit-8 (DOJINDO LABORATORIES, Kumamoto, Japan) according to the manufacturer’s instructions.