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Table 2 Box - Behnken experimental design with four independent variables (coded values) and the melanin production rate

From: Melanin-templated rapid synthesis of silver nanostructures

Run

A

B

C

D

Melanin yield (mg/ml)

Actual value

Predicted value

1

0

-1

1

0

1.90

1.94

2

1

0

0

-1

2.00

1.98

3

-1

0

0

1

2.00

2.06

4

0

0

1

-1

2.40

2.34

5

0

1

-1

0

2.10

2.12

6

1

-1

0

0

1.80

1.82

7

-1

-1

0

0

2.20

2.25

8

0

-1

-1

0

2.00

1.98

9

-1

1

0

0

1.90

1.91

10

0

1

0

-1

2.40

2.43

11

0

1

0

1

1.80

2.40

12

-1

0

-1

0

2.20

2.16

13

1

1

0

0

2.00

1.96

14

0

0

0

0

3.40

3.40

15

0

0

1

1

1.50

1.53

16

-1

0

1

0

2.00

1.95

17

0

0

-1

1

2.40

2.43

18

0

-1

0

1

2.10

2.14

19

0

1

1

0

1.60

1.63

20

-1

0

0

-1

2.60

2.65

21

0

0

0

0

3.40

3.40

22

0

0

0

0

3.40

3.40

23

0

0

0

0

3.40

3.40

24

1

0

0

1

2.20

2.16

25

0

-1

0

-1

2.30

2.22

26

0

0

0

0

3.40

3.40

27

0

0

-1

-1

2.10

2.10

28

1

0

-1

0

2.00

2.02

29

1

0

1

0

1.80

1.75

  1. A: Sucrose -1(20 g/l), 0 (25 g/l), +1(30 g/l); B: Tyrosine, -1(5 g/l), 0 (12.5 g/l), +1(20 g/l); C: Temperature, -1(20°C), 0(35°C), +1(50°C); D: Salinity, -1(1.5%), 0(2.5%), +1(3.5%).