Figure 3
Time-resolved high-frequency scanning acoustic microscopy for imaging and quantification of biofilm matrix. (A) A piezoelectric transducer transmits highly focused ultrasound beams at the sample under investigation. The ultrasound waves travel through the sample and the surrounding medium; reflected echoes are returned and recorded by the transducer. The echoes are resolved on the time axis. (B) The scanning acoustic microscopy unit is mounted onto an inverted light microscope allowing for a direct correlation of the same spatial regions in terms of optical (or fluorescence) and acoustic information assessment.