Figure 4

Protein origami: modular topological design of protein structure from a single polypeptide chain. A toolbox for constructing tetrahedron-like cage comprised of six orthogonal pairs of coiled-coil-forming peptides, two antiparallel- and four parallel dimers (orientation is denoted by arrow). Twelve peptides were concatenated in a defined order, separated by the tetrapeptide linker. The single polypeptide chain served as a building block that self-assembled into monomeric and asymmetric tetrahedron-like nanostructure [54].