Figure 3

(a) AFM height scan of mock – infected Vero cells at 36 h p.i. Scan size is 10 μm × 10 μm. In the mock-infected control sample, a distinct lack of filopodia and cytoskeleton formation is obvious. The bright spots depict high regions of the cells. (b) AFM amplitude scan of West Nile virus – infected Vero cells at 36 h p.i. Scan size is 50 μm × 50 μm. Arrows show the filopodia produced by these four infected cells. (c) AFM height scan of West Nile virus – infected Vero cells at 36 h p.i. of 6.9 μm × 6.9 μm size. Thin arrows show enveloped bags containing progeny West Nile viruses as they egress from host Vero cells. The arrowhead shows the filopodia formed in infected cells. Thick arrow shows the actin filaments near the budding enveloped bags. (d) The transmission electron micrograph shows similar image as in (c). The bags of virus (arrows) are seen exiting from the cell filopodia (filopodia).