Table 1 Primers Used for PCR
Amplicon – Primer | Sequence | 5' Label | Final Concentration |
|---|---|---|---|
HFE Exon 2 – forward | 5'-TCA GAG CAG GAC CTT GGT CTT TCC-3' | HEX | 0.4 μM |
HFE Exon 2 – reverse | 5'-CAT ACC CTT GCT GTG GTT GTG ATT-3' | N/a | 0.4 μM |
Amplicon – Primer | Sequence | 5' Label | Final Concentration |
|---|---|---|---|
HFE Exon 2 – forward | 5'-TCA GAG CAG GAC CTT GGT CTT TCC-3' | HEX | 0.4 μM |
HFE Exon 2 – reverse | 5'-CAT ACC CTT GCT GTG GTT GTG ATT-3' | N/a | 0.4 μM |