Figure 6
From: Cellular transfer and AFM imaging of cancer cells using Bioimprint
Bioimprint™ pattern transfer scheme for fabrication of negative cell replicas: Initially, all suspending medium is removed from cells attached to a Petri-dish, then a PDMS polymeric composite is poured over the cells and incubated. After curing, the hardened polymer is separated from the cells, washed and then the cell impression or mold is scanned by an AFM and digitally inverted to yield a positive replica matching the original cell orientation.