Figure 2

(a) Immunolabelled section of a resin-embedded Zea mays leaf using a CF1- antibody and subsequent signal detection by a combination of an anti-rabbit IgG- Biotin/quantum dot 565-streptavidin conjugate. Note the strongly labelled chloroplasts. White cross indicates position of the signal used for determination of the fluorescence spectrum. b) The graph shows the emission wavelengths versus their intensity in arbitrary units. (c) Comparison of stability of QD 565 versus Alexa 488 signals indicates a high stability of QD-based signals. Leaf sections immunolabelled with CF1-antibody detected by QD 565-streptavidin conjugate or Alexa 488-streptavidin conjugate and laser scanned 100 times.