Uranyl acetate negative stained transmission electron micrographs (TEM) of buckysomes. The scale bar in (A) is 500 nm and (B, C) is 100 nm. In micrographs (A, B) buckysomes were prepared in 10 mM citrate at pH 7.0 and in (C) Buckysomes were prepared in 1 × PBS buffer at pH 7.15. The concentration of AF-1 was 2 mg/mL and preparations were made at room temperature. Images are representative of 20–30 different areas on the grid.