Size characterization of buckysomes using Dynamic light scattering (DLS). Size distribution by DLS of buckysomes (2.0 mg/mL) prepared at pH 7.0 in 10 mM citrate buffer. The AF-1 dry powder was hydrated for 30 minutes at room temperature in the buffer and then extruded at 100°C using a 100 nm polycarbonate membrane. The average hydrodynamic diameter of the vesicles is 68 nm after 5 measurements. The correlation coefficient against time (μs) was fitted by a CONTIN algorithm in a multimodal fit. The size distribution ranges from 50 nm to 80 nm for the vesicles. The zeta potential in 10 mM citrate at pH 7.0 was -48 mV.