MTT and LDH assays. (A) MTT and (B) LDH assay showing the effects of buckysomes on cell viability and proliferation. Kidney, Liver, and Macrophage cells exhibited little differences when compared to PBS controls after exposure to AF-1 at different concentrations and analyzed for membrane integrity (LDH) as well as cellular proliferation (MTT). Samples A, B, C, D and E are 2 mg/mL AF-1, 0.2 mg/mL AF-1, 0.02 mg/mL AF-1, cells only, and control respectively. Cells were treated with 0.1% H2O2 for negative control of MTT and 0.9% Triton X-100 for positive control of LDH.