Fluorescent microscopy of human coronary artery endothelial cells incubated with 6-aminofluorescein-buckysomes for 18 hrs. The fluorescein coupled buckysomes were clearly cell associated, with no change in localization following several washes with PBS. Cells were fixed and counterstained with DAPI. (A) Superimposed image of fluorescein and DAPI emission. (B) Panel A superimposed with bright field image of cells. (C) Fluorescein emission at 520 nm. (D) DAPI emission at 461 nm. The scale bar for all panels is 50 μm.