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Figure 1 | Journal of Nanobiotechnology

Figure 1

From: Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay

Figure 1

Non-selective bioconjugation reaction scheme of carboxylated QDs (QD-COOH) to amine-containing proteins. This two-step reaction involves a) the activation of QD-COOH with EDC/sulfo-NHS, resulting in a semi-stable active ester (QD-NHS), and b) the nucleophilic reaction between the QD-NHS and amine-containing protein, forming a QD-protein conjugate via a stable amide bond.

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Journal of Nanobiotechnology

ISSN: 1477-3155

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