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Figure 11 | Journal of Nanobiotechnology

Figure 11

From: Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay

Figure 11

Overlapping electropherograms illustrating QD-rIgG (1) and antibody's respected immunogen QD-rIgG-HSA (2). IgG used for conjugation was rabbit anti-human albumin. Immunogen used was human serum albumin (HSA). CE buffer electrolyte used was 50 mM borate, pH 9.2. Gravity injection performed by elevating inlet capillary 7 cm for 5 s. Applied voltage for CE separation was 25 kV. Capillary temperature maintained at 20°C. Excitation source and detection wavelength was 473 nm and 620 nm, respectively.

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