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Figure 2 | Journal of Nanobiotechnology

Figure 2

From: Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay

Figure 2

Selective bioconjugation reaction scheme of amino QDs (QD-amine) to free sulhydryl-containing IgG antibodies. The reaction involves a) the mild reduction of IgG with cysteamine to yield partially reduced IgG antibody fragments (rIgG); b) the activation of QD-NH2 by nucleophilic reaction with NHS-moiety of sulfo-SMCC, resulting in maleimide-functionalized quantum dot (QD-maleimide); and c) the rIgG and QD-maleimide conjugation (QD-rIgG) via the formation of a thioether bond.

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