Figure 3From: Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassaySelective bioconjugation reaction scheme of hydrazide QDs (QD-hydrazide) to aldehyde-containing IgG antibodies (IgG-CHO). The reaction involves a) mild periodate oxidation of glycosylated IgG, yielding IgG-CHO; b) synthesis of QD-hydrazide via derivatization of QD-COOH with EDC/ADH; and c) conjugation of QD-hydrazide with IgG-CHO via formation of hydrazone linkage to yield QD-IgG.Back to article page