Antagonism by an A
AR antagonist of [35S]GTPγS binding induced by compound 17 . Compound 17 was incubated with increasing concentrations of A1 antagonist DPCPX, [35S]GTPγS, and a CHO A1 membrane suspension. The amount of [35S]GTPγS bound was measured, and the results were interpreted with Prism software. The results shown are means ± S.E.M. of three independent experiments.