AFM images. AFM images of substrates used in the experiment. Horizontal and vertical scale: 50 × 50 μ m2, 150 nm (A) TCPS. (B) Coverslip surface coated with ISA23-75. (C) Coverslip surface coated with AGMA1-75. The white box highlights a region where the thin hydrogel layer detached from the substrate, assuming a volcano-like appearance.