Table 2 Fraction of bound MNP*SAV2 to biotin-agarose beads after an incubation time tI in different suspension media estimated by fitting the MRX-curves by (2). The control sample means that SAV-molecules of the MNP*SAV2 are saturated with free biotin before incubation with analyte suspension.
From: Quantification of specific bindings of biomolecules by magnetorelaxometry
| Â | Â | Fraction of bound MNP | ||
|---|---|---|---|---|
| Â | Â | Â | Agarose beads | |
Medium | tI min | no analyte | Control | Binding |
BSA-B | 200 | 0.018 ± 0.006 | 0.054 ± 0.007 | 0.61 ± 0.04 |
serum tempered | 200 | 0.005 ± 0.008 | 0.117 ± 0.012 | 0.61 ± 0.04 |
serum | 200 | 0.011 ± 0.008 | 0.114 ± 0.009 | 0.87 ± 0.06 |