Figure 6

Dystrophin induction in TA muscles of mdx mice 3 weeks after intramuscular injections of AO uwith and without polymer carriers. Muscles were injected on days 0, 3, and 6 and harvested at 3 weeks after the initial injection. Dystrophin immunolabeling of TA muscle cross-sections at two different magnifications and H&E staining of serial sections are shown for (a-a") normal, (b-b") mdx untreated, (c-c") mdx injected with AO alone, (d-d") mdx injected with PEG-PEI-AO polyplex, and (e-e") mdx injected with PLGA (17 kDa) nanospheres encapsulated with the PEG-PEI-AO polyplex.