Figure 7

Comparison of the number of dystrophin-positive fibers in TA muscles of mdx mice at 3 weeks after 3 intramuscular injections of PEG-PEI-AO polyplex and PLGA (17 kDa) nanospheres encapsulated with the PEG-PEI-AO polyplex. Results are also shown for injections of AO alone and untreated mdx muscles which are known to contain a small number of revertant fibers. All treated muscles were given 5 μg AO per injection. Both PLGA encapsulated polyplex and unencapsulated polyplex showed a significantly higher number of dystrophin positive fibers compared to AO alone injected or untreated mdx muscle (*P < 0.05). No statistical difference in dystrophin positive fibers was observed between untreated muscle and AO alone injected groups. Fiber counts were determined from four independently treated muscles in each group (N = 4).