Figure 4
Effects of metallic nickel particles on caspase-8, Fas, FADD, DR3, DR6, TNF-R2, p-Akt, DISC, lamin A, beta-actin, BID, Bcl-2, and BAX. Cells were treated with 20 μg/cm2 metallic nickel particles for 30, 60, 120, and 180 min. Expressions of caspase-8, Fas, FADD, DR3, DR6, TNF-R2, and p-Akt were analyzed by western blot (A). To investigate the formation of DISC, IP western blot was used (B). Cells were treated with metallic nickel particles for 1, 3, 6, and 8 h. Effects of metallic nickel particles on lamin A, beta-actin, and Bcl-2 family were detected by western blot (C and D).