Figure 2

The effect of co-transfecting plasmid DNA and laser generated gold nanoparticles on the bioactivity of GFP-HMGB1 fusion protein. Images A to I (vertical) show a 100 fold magnification and B to J (vertical) a 400 fold magnification. Images A and B represent the positive control I: a transient transfection of MTH53a cells by Fugene HD reagent with the eGFP-C1-HMGB1 plasmid without Au-NP incubation. Cells in images C to J are treated like control I but include incubation of the plasmid with 14 nm sized Au-NP (C and D), 24 nm sized Au-NP (E and F), 59 nm sized nanoparticles (G and H) and 89 nm sized Au-NP (I and J), respectively. Image K and L represent the negative controls II and III. M and N are full color images of DAPI and GFP fluorescence.