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Figure 3 | Journal of Nanobiotechnology

Figure 3

From: Co-transfection of plasmid DNA and laser-generated gold nanoparticles does not disturb the bioactivity of GFP-HMGB1 fusion protein

Figure 3

Au-NPs/DNA and HMGB1 protein mobility shift assay. M: GeneRuler 1 kb Plus (Fermentas), lane 1: 170 ng plasmid (NcoI digested); lane 2: 170 ng plasmid (NcoI digested) and 1.5 μg HMGB1; lanes 3-6: 170 ng plasmid 170 (NcoI digested) and 1.5 μg HMGB1 in 0.1 nM, 0.5 nM, 1.0 nM and 2.5 nM AuNPs suspensions, size dh 24 nm; lane 7: 170 ng plasmid (NcoI) digested and 1.5 μg HMGB1 and 90 ng pure Au suspension, size dh 24 nm; lanes 8-11: 170 ng plasmid (NcoI digested) and 1.5 μg HMGB1 in 0.1 nM, 0.5 nM, 1.0 nM and 2.5 nM AuNPs suspensions, size dh 59 nm; lane 12: 170 ng plasmid (NcoI) digested and 1.5 μg HMGB1 and 50 ng pure Au suspension, size dh 59 nm.

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