Figure 3
Repeated observation of a cell from a mixed neural-glial culture. Six scans of a cultured neural cell performed within 202 min. (A)-(F) Three dimensional plots of the data. Scan dimensions are indicated in (D), height indicated by the color gradient at the top of frame (A), main scanning direction is indicated by the arrow at the upper left corner in (E). Insets in (A) and (B) display a magnification of the area marked by the dashes with increased contrast, white scale bar indicates 3.5 μm. Arrows mark different cell parts that changed during the time of observation. Red: Old main process of the observed cell, orange: new leading process, blue and green: upper and lower process of the 2nd cell, respectively, yellow: terminal fan-formed structure of the process marked green, cyan: novel process, white: newly emerging membrane protrusions. White double-headed arrow in (D) marks a change in position of the cell body. Lateral step size was 500 nm, vertical step size was 100 nm, frame acquisition time was about 30 min.