Figure 3

Effect of VEGF and IL-1β on endothelial cell permeability. PRECs were grown to confluent monolayers on porous membranes (12-well transwell insert plate) and treated with various concentrations of VEGF (A) and IL-1β(B). The flux of RITC-dextran from the upper to the lower chamber was measured after 6 h of treatment. Both VEGF-and IL-1β-induced endothelial cell permeability in dose-dependent manner. Values are expressed in relative fluorescence counts (RFUs) as mean ± SEM, with each condition performed at least in triplicate (n = 3, *P < 0.05 vs control). The figure is representative of three experiments with similar results.