Figure 4

Effect of various concentration of Ag-NP on VEGF and IL-1β-induced endothelial cell viability. PRECs were seeded in 96-well plate at a density of 2 × 10³ cells/well and grown to confluence. After reaching confluence, PRECs treated with indicated concentration of Ag-NP in presence of 25 ng/ml VEGF (A) and presence of 10 ng/ml IL-1β (B) for 24 h and the cell viability was measured by MTT assay. 100 nM Ag-NP significantly reduced the VEGF and IL-1β-induced cell proliferation (2 fold). Data are mean ± SEM representing similar results was obtained in three independent experiments (n = 3,*P < 0.05 vs VEGF and IL-1β treatment, **P < 0.01 vs VEGF and IL-1β treatment).