Figure 2
From: Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo
UV-inducible and wortmannin-sensitive translocation of QD-Akt-PH-EGFP conjugates to the membrane. (a) Akt-PH-EGFP protein fusions and Akt-PH-QDot585 conjugates translocate to the cell membrane upon exposure of injected Xenopus embryos to UV radiation. Live Xenopus embryos injected as described were imaged on a Zeiss Axioimager to visualize the localization of Akt-PH-EGFP and Akt-PH-QD conjugate before and after exposure to UV radiation for 5 min. Both Akt-PH-EGFP and Akt-PH-QD conjugates translocate to the cell membrane following brief exposure to UV radiation. (b) Translocation of Akt-PH-QD conjugates (QDot655) to the cell membrane is Wortmanin sensitive. Live Xenopus embryos were imaged on a Zeiss Axioimager to visualize the localization of Akt-PH-QD conjugate before and after treatment with Wortmannin (400 nM), a PI3-K inhibitor, for 1 hour. The Akt-PH QD conjugates become diffusely localized in the cytosol after treatemnt.