Figure 3

QD-Akt-PH conjugates are resistant to photobleaching, unlike Akt-PH-EGFP fusions. Fluorescence images of stage 10 Xenopus embryos microinjected with the probe (I_C-QD₅₂₅) and with RNA encoding the target protein (Akt PH-EGFP-I_N), both shown in green since their emission spectra are closely matched. Embryos were exposed to continuous excitation (~ 480 nm) for > 20 min. This led to gradual loss of the EGFP signal but did not affect the QDot₅₂₅ signal.