Figure 1
From: Immobilization of different biomolecules by atomic force microscopy
Illustration of the spotting process. The first spot is generated by addressing the spotted neutravidin with biotinylated oligonucleotides. All following spots can be created by repeated spotting and addressing without replacing the surface. Here, the whole cycle is completed after two passes. By hybridization with complementary ssDNA which is fluorescently labeled the array can be visualized.