FhuA Δ1-160 variant reconstituted in liposomes | Position(s) of Lys→Ala Substitution(s) | TMB conversion [nM]/s | *True averaged TMB conversion [nM]/s | **TMB conversion ratio | |
---|---|---|---|---|---|
Controls | Lacking FhuA Δ1-160 | - | 13 ± 2 | - | 1 |
Unlabeled FhuA Δ1-160 | - | 102 ± 5 | 89 | 7.9 | |
Fully labeled FhuA Δ1-160 starting variant | - | 15 ± 4 | 2 | 1.2 | |
Subset A | K167A | 167 | 59 ± 2 | 46 | 4.5 |
K344A | 344 | 52 ± 1 | 39 | 4 | |
K364A | 364 | 20 ± 3 | 7 | 1.5 | |
K537A | 537 | 76 ± 3 | 63 | 5.9 | |
K556A | 556 | 97 ± 4 | 84 | 7.5 | |
K586A | 586 | 14 ± 1 | 1 | 1.1 | |
Subset B | K167 | 344, 364, 537, 556, 586 | 22 ± 2 | 9 | 1.7 |
K344 | 167, 364, 537, 556, 586 | 23 ± 1 | 10 | 1.8 | |
K364 | 167, 344, 537, 556, 586 | 30 ± 3 | 17 | 2.3 | |
K537 | 167, 344, 364, 556, 586 | 21 ± 3 | 8 | 1.6 | |
K556 | 167, 344, 364, 537, 586 | 16 ± 1 | 3 | 1.2 | |
K586 | 167, 344, 364, 537, 556 | 35 ± 2 | 22 | 2.7 |