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Table 1 Average TMB conversions in liposomes.

From: Molecular understanding of sterically controlled compound release through an engineered channel protein (FhuA)

 

FhuA Δ1-160 variant reconstituted in liposomes

Position(s) of Lys→Ala Substitution(s)

TMB conversion [nM]/s

*True averaged TMB conversion [nM]/s

**TMB conversion ratio

Controls

Lacking FhuA Δ1-160

-

13 ± 2

-

1

 

Unlabeled FhuA Δ1-160

-

102 ± 5

89

7.9

 

Fully labeled FhuA Δ1-160 starting variant

-

15 ± 4

2

1.2

Subset A

K167A

167

59 ± 2

46

4.5

 

K344A

344

52 ± 1

39

4

 

K364A

364

20 ± 3

7

1.5

 

K537A

537

76 ± 3

63

5.9

 

K556A

556

97 ± 4

84

7.5

 

K586A

586

14 ± 1

1

1.1

Subset B

K167

344, 364, 537, 556, 586

22 ± 2

9

1.7

 

K344

167, 364, 537, 556, 586

23 ± 1

10

1.8

 

K364

167, 344, 537, 556, 586

30 ± 3

17

2.3

 

K537

167, 344, 364, 556, 586

21 ± 3

8

1.6

 

K556

167, 344, 364, 537, 586

16 ± 1

3

1.2

 

K586

167, 344, 364, 537, 556

35 ± 2

22

2.7

  1. Lambert Beer law was used with an extinction coefficient of 3.9 × 10-4 M-1 cm-1 for the first TMB oxidation product. Two subsets (A & B) of FhuA Δ1-160 variants were apart from controls analyzed. FhuA Δ1-160 variants of subset A contain a single lysine to alanine substitution while subset B contain five lysine to alanine substitutions. All FhuA Δ1-160 variants are pyridyl-labeled except two controls (liposome lacking FhuA Δ1-160 and the unlabeled FhuA Δ1-160). "*": The true TMB conversion is calculated from the TMB-conversion of FhuA Δ1-160 variant subtracted by the TMB conversion of the background lacking FhuA Δ1-160; "**": TMB conversion ratio represents a ratio between TMB conversions of pyridyl-labeled FhuA Δ1-160 variants and the liposome control lacking FhuA Δ1-160.