Table 2 Primers used for Site Directed Mutagenesis (SDM)
Sequence Name | Sequence 5' to 3' |
|---|---|
FhuA Δ1-160 K167A Fwd | CCGCTGAAAGAAGTTCAGTTTGCGGCCGGTACTGACAGCC |
FhuA Δ1-160 K167A Rev | GGCTGTCAGTACCGGCCGCAAACTGAACTTCTTTCAGCGG |
FhuA Δ1-160 K344A Fwd | GGCCATTATCTGGCACGTGCGTACGTCGTTGATGATGAGAAG |
FhuA Δ1-160 K344A Rev | CTTCTCATCATCAACGACGTACGCACGTGCCAGATAATGGCC |
FhuA Δ1-160 K364A Fwd | GATACCCAGTTGCAGAGCGCGTTTGCCACTGGCGATATCG |
FhuA Δ1-160 K364A Rev | CGATATCGCCAGTGGCAAACGCGCTCTGCAACTGGGTATC |
FhuA Δ1-160 K537A Fwd | GCAGTATGAAGTCGGCGTGGCGTATGTACCGGAAGATCG |
FhuA Δ1-160 K537A Rev | CGATCTTCCGGTACATACGCCACGCCGACTTCATACTGC |
FhuA Δ1-160 K556A Fwd | GCCGTGTATAATCTCACTGCGACCAACAACCTGATGGCGG |
FhuA Δ1-160 K556A Rev | CCGCCATCAGGTTGTTGGTCGCAGTGAGATTATACACGGC |
FhuA Δ1-160 K586A Fwd | CGTAGAAATCGAAGCGGCGGCGGCGCTGTCGGCGAG |
FhuA Δ1-160 K586A Rev | CTCGCCGACAGCGCCGCCGCCGCTTCGATTTCTACG |