Figure 4

Detection of angiogenesis- and inflammation-inducing factors by Western blot analysis. HCT116 cells were treated with empty PLGA NPs (control), DCPIP, and DCPIP NPs for 48 hr, then, aliquots of 35 μg of whole cell protein were subjected to analysis for detection of VEGF, COX-2, IL-6, IL-8 and β-actin proteins. β-actin was used as a cell internal protein marker. An additional control included untreated (CTL) cells.