The diameter of PLGA nanoparticles can be controlled by the flow rates and PLGA concentrations. (A) SEM images and diameters of PLGA nanoparticles fabricated at dispersing flow rates of 35 ml/min, 50 ml/min, and 80 ml/min. (B) SEM images and diameters of PLGA fabricated at PLGA concentrations of 10 mg/ml, 20 mg/ml, and 40 mg/ml. Diameters were measured by using ImageJ. For each sample, the mean diameter was calculated based on the measurements of 100 randomly chosen particles. Samples are imaged without filtration.