TCRV replication following exposure to Ag-NPs. TCRV was treated with uncoated and PS-coated 10 and 25 nm Ag-NPs for 1 hour. Treated or control virus suspensions were used to infect Vero cells for 8 days. Progeny virus was recovered from the cell culture supernatant and was quantified using a standard tissue culture infectious dose assay. Progeny virus titers are expressed as the mean log10 TCID50/mL +/- S.E.M. (T = concentration of nanoparticles was toxic at this dose; *p < 0.05; student's t test; n = 6).