Figure 3

Confocal imaging of untreated and Ag-NP-treated TCRV in Vero cells. TCRV was labeled with a fluorophore that excites at a wavelength of 488 nm and was treated with Ag-NPs for 1 hour. Treated or control virus was used to infect Vero cells for 4 hours. The supernatant was removed and the cells were washed 2 times with PBS to remove non-adherent virus and were fixed in 3% paraformaldehyde. The nuclei were stained with Hoechst (blue) and the images were taken using spinning disc Confocal microscopy with the pictures representative of collapsed z-stacks of sections through the cell (15 sections). (a) Vero cells alone (b) TCRV in Vero cells (c) TCRV + 10 nm Ag 50 μg/ml (d) TCRV + 10 nm Ag 10 μg/ml (e) TCRV + 10 nm PS-Ag 50 μg/ml (f) TCRV + 10 nm PS-Ag 10 μg/ml (g) TCRV + 25 nm Ag 50 μg/ml (h) TCRV + 25 nm Ag 10 μg/ml (i) TCRV + 25 nm PS-Ag 50 μg/ml (j) TCRV + 25 nm PS-Ag 10 μg/ml (representative picture of 3 separate trials).