Skip to main content
Figure 2 | Journal of Nanobiotechnology

Figure 2

From: Development of PEGylated PLGA nanoparticle for controlled and sustained drug delivery in cystic fibrosis

Figure 2

Release kinetics of PLGA-PEG nanoparticles shows sustained release and drug activity overtime. A) Release kinetics of nile red from PLGA-PEG nanoparticles (n = 3) was quantified by recording absorption of released dye at 525 nm. We observed a sinusoidal-like, sustained release of the dye from day 1 to 15, with a maximum release at day 10. Triplicate samples are shown by different symbols. B) We quantified the release kinetics of PS-341 from PLGA-PEG and DSPE-PEG, once daily for 7-days, using the proteasomal activity assay. We recorded proteasome inhibitory activity (Relative Luminescence Units, RLU) of room temperature incubated PLGA-PEGPS341 and DSPE-PEGPS341 nanoparticles for day 1 to 7, and observed more effective and sustained drug activity of PS341 from PLGA-PEG compared to DSPE-PEG. C) We compared the efficacy of PLGA-PEGPS341 drug delivery in CFBE41o- cells as compared to PS-341 by Proteasome-Glo Chymotrypsin Cell Based Assay (Promega). We observed a significantly enhanced decrease in proteasome activity when using the PLGA-PEG mediated PS341 delivery as compared to the PS341 treatment at similar concentrations. The PLGA-PEG nanoparticle system provides sustained release and drug activity, and enhances therapeutic effectiveness.

Back to article page