Figure 6

Treatment with PLGA-PEG^PS341 attenuates NFκB and IL-8 promoter activities. CFBE41o- cells were treated for 24 hours with 100 ng PLGA-PEG^PS-341 and transfected with NFκB, IL-8 and/or renila luciferase reporter plasmids. After six hrs of transfection, TNF-α (10 ng/ml) was used to induce proinflammatory signaling overnight. NFκB- and IL-8- firefly and renila luciferase activities were quantified using the Dual Luciferase^® Reporter Assay System. We observed that treatment with the 10 μl of PLGA-PEG^PS341 (10 ng/μl) significantly decreases TNF-α induced A) NFκB and B) IL-8 luciferase activities (*p < 0.05). Data is shown as RLU (Relative Luminescence Intensity) of firefly luciferase promoter activity normalized to renilla luciferase internal control. The data verifies the efficacy of PLGA-PEG mediated drug delivery and activity.