Figure 8
Treatment with PLGA-PEGPS341 attenuates Pa -LPS induced proinflammatory response and neutrophil levels. The Cftr-/- mice (n = 3, each group) were treated with Pa-LPS and/or PLGA-PEGPS341. Control, untreated group, was treated with 10 μl saline. The bronchoalveolar lavage fluid (BALF) cytokine and myeloperoxidase (MPO), levels were quantified by sandwich ELISAs. The treatment with the PLGA-PEGPS341 significantly decreased Pa-LPS induced A) IL1-β, B) IL-6 and C) MPO levels confirming that PLGA-PEG mediated PS-341 delivery controls Pa-LPS induced inflammatory response and neutrophil chemotaxis (*p < 0.05). The data verifies the efficacy of PLGA-PEG mediated PS-341 drug delivery in controlling Pa-LPS induced lung disease.