Figure 7
From: The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture
Detection of O 2 - • by the NBT assay in cell suspension cultures. (A) Treated with NBT. (B) Treated 20 minutes at 45°C and NBT exhibiting blue formazan spots due to O2- • production (indicated by arrows). (C) Treated with 100 nM QDs and stained with NBT showing no blue formazan spots. (D) Treated with 100 nM QDs for 48 hours and NBT and (E) treated with 100 nM QDs for 72 hours and NBT confirming that there is no O2- • production. All samples were visualized 4 hours after the NBT addition in bright field. Scale bar = 50 μM.