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Figure 8 | Journal of Nanobiotechnology

Figure 8

From: The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture

Figure 8

General oxidative stress assay in cell suspension cultures. (A) Treated with H2DCFDA showing a basal level of fluorescence. (B) Treated 20 minutes at 45°C and H2DCFDA exhibiting a fluorescence increase due to an oxidative stress. (C) Treated with 100 nM QDs and H2DCFDA. (D) Treated with 100 nM QDs for 48 hours and H2DCFDA and (D) treated with 100 nM QDs for 72 hours and H2DCFDA. Scale bar = 50 μM. (F) graphic representation of mean fluorescence intensity in A-E. Black bars represent the mean of pictures from control cultures (cells + H2DCFDA) and grey bars the QD treated cultures in the three exposure times performed. Data represent mean from one experiment (n = 6) + SD. Columns with * indicate a significant difference from the control value with p < 0.001 (ANOVA).

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