Figure 9

Oxidative stress dose response assay. Cell suspension cultures treated with QDs and H₂DCFDA: (A) 1 nM QDs, (B) 5 nM QDs, (C) 10 nM QDs, (D) 20 nM QDs, (E) 40 nM QDs, (F) 60 nM QDs, (G) 100 nM QDs, (H) 120 nM QDs, (I) 180 nM QDs. Scale bar = 50 μM. (J) graphic representation of mean fluorescence intensity of pictures from cultures subjected to the different treatments. Data representing means from two independent experiments + SD (n = 6 in each experiment), except for the conditions 120 nM and 180 nM which represent one experiment (n = 7). Columns with * indicate a significant difference from the control value with p < 0.001 (ANOVA). Fluorescence intensity in cultures treated with QDs shows a linear behaviour represented by Y = 0,1581x + 9,6342 and R² = 0,9735. The H₂DCFDA assay was performed 48 hours after the QD addition.