Figure 1

Identification of lipids and proteins bound to MWCNTs. A) TLC separation of bound lipid components. From left to right: Lipids from pure Curosurf (CS), lipids bound to the P-MWCNT, MWCNT-NH₂, MWCNT-COOH. Abbreviations for the lipids: TG Triglyceride, PG Phosphatidylglycerol, PE Phosphatidylethanolamie, PS Phosphatidylserine, PI Phosphatidylinositol, PC Phosphatidylcholine, SM Sphingomyelin, PIP Phosphatidylinositolphosphate. Lipid classes were allocated by comparisons to the literature [37, 61] and in addition three of the most abundant lipids (Phosphatidylcholine, Phosphatidylethanolamine, Phosphatidylglycerol) were confirmed by the use of standards (lanes 5-7). The arrow points to the front of the first solvent. B) Lipids bound to P-MWCNT incubated in Curosurf and post-incubated in Roswell Park Memorial Institute Medium (RPMI) and in blood plasma respectively. RPMI which was used as a control for cell culture medium did not alter the lipid patterns which were obtained by pure Curosurf incubation. The arrow points to the front of the first solvent. C) Plasma proteins adsorbed on the different functionalized MWCNTs separated by SDS-PAGE (left part) and quantified by densitometry (right part). 1. Alpha-2-macroglobulin precursor; 2. Complement factor H; 3. Inter-alpha (globulin) inhibitors H1, H2, H4, Complement component 7, Plasminogen; 4. Gelsolin isoform c, Cadherin-5; 5. Coagulation factor XI; 6. Keratin 6A. D) Effect of a Curosurf pre-incubation (P-MWCNT+CS) on the protein adsorption pattern. Arrows point to characteristical bands. 1. Apolipoprotein A (precursor), Apolipoprotein B (precursor); 2. Unknown; 3. Ceruloplasmin; 4. Unknown; 5. Fibrinogen beta chain.