Figure 8

Dynamic distribution of LPS+Gd-DOTA in the inner ear after intracochlear delivery in closed perilymphatic compartments demonstrated by MPR single view of T1-weighted 3D MRI. 1 mM nanoparticles containing 500 mM Gd-DOTA were administered. In the cochlea, LPSG+Gd-DOTA filled SM at the beginning, but were cleaned from SM after 56 m (A, B, and C). In the vestibule, LPS+Gd-DOTA retained in the proximal locations of SCCs, but distributed to the distal parts of SCCs at 56 m post-injection (D, E, F). 1^st: the basal turn; 2^nd:the second turn; 4 m, 12 m, and 56 m: 4 minutes, 12 minutes, and 56 minutes; Mod: modiolus; MPR: multiplanar reconstruction; SSCs: semicircular canals; SM: the scala media; SM-h1st: SM of the higher basal turn; SM-h2nd: SM of the higher second turn; ST: the scala tympani; SV: the scala vestibuli; Vest: vestibule. Scale bar = 500 μm.