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Table 3 Dependency of Staphylococcus aureus tm on keratin-stabilized Np (freeze-dried) and associated probabilities (P+) that the changes in tm are due to perturbations in the CI (~ 8 × 103 CFU mL-1) in the presence of the Nps.

From: Antimicrobial activity of spherical silver nanoparticles prepared using a biocompatible macromolecular capping agent: evidence for induction of a greatly prolonged bacterial lag phase

per well

tm (hrs)

  

μ gNp

μ g keratin

Exp: 1

2

3

avg

P+, avg

Tcorr, avg(hrs)

0

0

6.74

6.13

6.89

6.59

a

1

1.11

5

0

8.72

7.18

8.52

8.14

a

1

2.66

10

0

13.3

11.4

12.3

12.3

b

0.798

6.83

15

0

13.4

12.8

13.9

13.4

b

0.644

7.91

20

0

15.7

14.7

15.6

15.4

c

0.0995

9.89

25

0

16.8

15.7

16.3

16.2

c

0.100

10.8

30

0

19.3

18.2

17.8

18.4

d

0.00551

13.0

35

0

23.5

20.3

21.4

21.7

e

0.00163

16.3

40

0

24.9

21.7

24.8

23.8

f

0.0000628

18.3

45

0

27.1

26.9

27.3

27.1

g

0.00000288

21.6

50

0

27.6

28.4

26.8

27.6

g

0.00000165

22.1

0

10

6.65

6.11

6.89

6.55

a

1

1.08

0

25

6.95

6.20

6.69

6.62

a

1

1.14

0

50

6.69

6.00

6.73

6.47

a

1

1.00

  1. Averages associated with different letters are significantly different at the p = 0.05 level. There is no significant effect of the keratin alone on tm. The 5 μg Np level is equivalent to ca. 2.6 × 10-7 M.