Figure 1
From: Labelling of live cells using fluorescent aptamers: binding reversal with DNA nucleases
An overview of the reversible aptamer labelling of live cells. Intact cells are labelled with biotin-aptamer conjugate, followed by streptavidin-Qdot conjugates. Next, the Qdot-labelled aptamer cells are incubated with nuclease (DNase treated cells), this removes aptamers from the cells. The fluorescence intensities of intact cells, aptamer-labelled cells and DNase treated cells are compared using flow cytometery; possible cell damage is evaluated by measuring cell growth rates.