Figure 7

A scheme showing protein immobilization and capture on gold nanoparticles (GNPs). (A-E), GST-derivatised GNPs. (A) The addition of extra GST does not result in any detectable interaction. (B) The addition of GST-SNAP25 fusion protein does not result in any detectable interaction. (C) The addition of SNAP25 does not result in any detectable interaction. (D) The addition of GST-NL fusion protein does not result in any detectable interaction. (E) The addition of NL fusion peptide does not result in any detectable interaction. (F-G) GNPs derivatised with GST-NL fusion protein. (F) The addition of GST-SNAP25 fusion protein results in specific interaction and the formation of the tight tetra-helical assembly. (G) The addition of SNAP25 construct results in specific interaction and the formation of the tight tetra-helical assembly. (H-I) GNPs derivatised with GST-SNAP25 fusion protein. (H) The addition of GST-NL fusion protein results in specific interaction and the formation of the tight tetra-helical assembly. (I) The addition of NL fusion peptide results in specific interaction and the formation of the tight tetra-helical assembly. In all panels, the filled circle symbolizes a gold nanopartice, a grey-filled arch denotes a GST protein, red coloured cylinders represent the two helices based on the SNAP25 protein sequence, blue coloured cylinders indicate a NL fusion peptide.