Sub-cellular internalization of nanomaterials in human cultured cells. (A) Cell-cycle arrest and cell viability were tested by Flow cell cytometry data of HEK-293 cells obtained after incubation in culture media containing different concentration of nanoparticles C-16. (B) The confocal images of HeLa cells after incubation at 37°C and 4°C in nanoparticles (C-16 containing media) (C) cells pre-treated with 0.45 M sucrose and K+ - depleted medium, (D) after pre-treatment with NaN3 respectively (E) Flow cytometry data of HeLa cells with no pre-treatment and pre-treated with filipin and nystatin were presented in a bar diagram. Cholera toxin B (Black) and C-18 nanoparticle (blank) uptake was shown. Scale 50 μm (Cells).