Figure 8

Nuclear fragmentation and reduction of actin filaments in MCF-7 breast carcinoma cells 48 hours after treatment. Cells were stained with DAPI (4',6-diamidino-2-fenilindol) to visualize the nucleus (in blue) and with Phalloidine-Alexa Fluor 488 to visualize actin (in green). (A, B) control (cells without treatment); (C, D) cells treated with 50 μM and (E, F) with 500 μM of Rh₂(H₂cit)₄; (G, H) cells treated with 10 nM paclitaxel for 2 h. Arrows and arrow heads indicate nuclear fragmentation and chromatin condensation, respectively. Phase-contrast images are presented for validation of fluorescence (Figure 8B, D, F, H).